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Journal of the Korean Society of Neonatology 2001;8(1):133-140.
Published online May 1, 2001.
Early Prediction of Hypoxic-Ischemic Brain Damage in Newbon Rats Using Proton Magnetic Resonance Spectroscopy and Neuroprotective Effect of EGb 761.
Sung Jong Park, Hye Sun Yoon, Keun Ho Lim, Jung Hee Lee, Ki Soo Kim, Soo Young Pi
1Department of Pediatrics, College of Medicine, Ulsan University,Ulsan University Hospital, Ulsan.
2Department of Pediatrics, Eulji Medical College, Seoul NMR Laboratory, Asan Institute for Life Sciences.
3Department of Pediatrics, College of Medicine, Ulsan University, Asan Medical Center, Seoul, Korea.
저산소성 허혈성 뇌손상을 유도한 신생 백서에서 뇌손상의 조기 예후 예측 도구로써 수소 자기공명분광의 유용성 및 EGb 761의 뇌보호 효과
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This study was designed to evaluated the relationship between the morphological changes of hypoxic-ischemic injured brain and the magnetic resonance spectroscopic (MRS) findings, and the efficacy of Egb 761, a free radical scavenger, as a neuroprotective agent for hypoxic-ischemic brain injury.
Seven-day old Sprague-Dawley rat pups were used. Right common carotid artery was ligated under halothene anesthesia. After a recovery period of 3 hours, they were divided into two groups : for group 1, EGb 761 100 mg/kg (treatment group) and for group 2, normal saline (control group) were given intraperitoneally. Both groups were exposed to 8% oxygen at 37degrees C for 90 minutes. Sixty-four rat pups (30 control, 34 treatment group) were examined with localized 1H MRS on days 1 and 7 after the hypoxic insult. One day 14, the degree of brain injury was scored by morphological changes.
The 1H MRS obtained on day 1 after the hypoxic insult showed increased Lip/NAA and Lip/Cr ratios in the right cerebral hemisphere in comparison with those in the left hemisphere (P<0.01). The degree of morphological changes of the brain injury on day 14 correlated with both Lip/NAA and Lip/Cr ratios obtained on days! and 7 after the hypoxic insult [r=0.410, 0.457 on day 1, and r=0.749, 0.720 on day 7, respectively (P< 0.01)]. The therapeutic potential of EGb 761 for the hypoxic-ischemic brain seemed insignificant, as was evident from no differences between the control group and the EGb 761 treatment group in the Lip/NAA and Lip/Cr ratios as well as in morphological changes.
Early changes of Lip/NAA and Lip/Cr ratios in the 1H MRS seems to be related to the degree of morphological changes in the rat brain of hypoxic-ischemic injury. Therefore, the increased lipid content of brain observed by 1H MRS can be used as a marker to predict the degree of the hypoxic-ischemic injury. The neuroprotectiveeffect of EGb 761 in hypoxic-ischemic brain injury is insignificant.
Key Words: Hypoxic-ischemic brain injury; 1H magnetic resonance spectroscopy; EGb 761
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