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Journal of the Korean Society of Neonatology 2007;14(2):162-169.
Published online November 1, 2007.
Expression of Fibroblast Growth Factor Receptor mRNAs according to Administration of Geneticin in Hypoxic Neuron Cell Culture.
Kyung Hye Keum, Eok Su Seo, Woo Taek Kim
1Department of Ophthalmology, School of Medicine, DongGuk University, Kyeong-Ju, S. Korea.
2Department of Pediatrics, School of Medicine, Catholic University of Daegu, Daegu, S. Korea. wootykim@cu.ac.kr
Expression of Fibroblast Growth Factor Receptor mRNAs according to Administration of Geneticin in Hypoxic Neuron Cell Culture
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Abstract
PURPOSE
Recently, Geneticin (G418) were known to exert neuroprotective effects in the hypoxic-ischemic (H-I) brain injury, but the mechanism is still unclear. The roles of fibroblast growth factor (FGF) and FGF receptor (FGFR) ware not well known in the H-I brain injury. We investigated the neuroprotective effects of systemically administrated Geneticin through the regulation of FGFR following the H-I brain injury METHODS: The cortical neuron cell culture of Spague-Dawley (SD) rat embryo brain (E18) was done in a hypoxic incubator. The cultured cells were divided three groups: a normoxia group, a hypoxia group, and an Geneticin-treated group. After verifying the desired amount of cellular injury in the hypoxia group, the Geneticin-treated group (after an H-I insult) was further divided into two groups. This produced four final groups: normoxia, hypoxia, and Geneticin-treated groups before H-I insult and a Geneticin-treated group after HI insult. The expression of FGFR-2 and FGFR-3 mRNA was measured using Northern blotting.
RESULTS
The expression of FGFR-2 and FGFR-3 mRNA was notably increased in the hypoxic group compared to the normoxic group. In both Geneticin-treated groups before and after a hypoxic insult, the expression of FGFR-2 and FGFR-3 mRNA was decreased.
CONCLUSION
It suggests that FGFR has an important role in hypoxic brain injury. Geneticin appears to exert a protective effect through down regulation of the expression of FGFR mRNA. However, more experiments are needed in order to demonstrate the usefulness of Geneticin as a preventative and rescue treatment for H-I brain injuries of neonatal brain.
Key Words: Geneticin (G418), Hypoxic-ischemic brain injury, Fibroblast Growth Factor Receptor (FGFR)
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