Neonatal Med Search

CLOSE


Neonatal Med > Volume 21(2); 2014 > Article
Neonatal Medicine 2014;21(2):144-150.
DOI: https://doi.org/10.5385/nm.2014.21.2.144    Published online May 30, 2014.
Intestinal Microbial Community Profiles of a Newborn Preterm Infant Using Pyrosequencing Analysis: Pilot Study.
Jong Geol Kim, Sung Keun Rhee, Young Don Kim
1Department of Microbiology, Chungbuk National University, Cheongju, Korea.
2Department of Pediatrics, Jeju National University School of Medicine, Jeju, Korea. ydkim@jejunu.ac.kr
Abstract
DNA pyrosequencing, one of the advanced methods for DNA sequencing, has been employed for phylogenetic analysis of bacterial communities using the conserved 16S rRNA gene. We performed a pilot study on a mother-neonate pair utilizing the DNA pyrosequencing assays to investigate the diversity of microbial communities in maternal amniotic fluid (AF), vagina, and rectum and newborn gastric fluid (GF) and stool. Phylum level analysis revealed that bacterial community was dominated by Firmicutes (63.2%) in maternal feces, and Actinobacteria (84.9%) in maternal vaginal swab. The bacterial communities in both the AF and GF were dominated by Proteobacteria (67.8%). Interestingly, the bacterial community in the newborn's meconium was quite similar to that in the AF. However, the composition of the bacterial community in newborn's feces was different on day 14 and dominated by Firmicutes (91.1%). Genus-level analysis revealed that the bacterial community in maternal feces was dominated by Anaerococcus (19.5%) and Prevotella (18.7%), whereas that in the maternal vaginal swab was dominated by Atopobium (83.6%). The bacterial communities in both the AF and GF were dominated by Sphingomonas (38.5%). The bacterial community in the newborn's meconium was quite similar to that in the AF, which was dominated by Sphingomonas (45.2%). However, the composition of bacterial community in the newborn's feces on day 14 was relatively different. Future studies with a large number of infants are needed to determine the factors involved in the changing profile of newborn's fecal bacterial communities.
Key Words: Pyrosequencing, Amniotic fluid, Gastric fluid, Microbiota, Gut colonization
TOOLS
Share :
Facebook Twitter Linked In Google+ Line it
METRICS Graph View
  • 0 Crossref
  •    
  • 1,544 View
  • 13 Download


ABOUT
ARTICLE CATEGORY

Browse all articles >

BROWSE ARTICLES
AUTHOR INFORMATION
Editorial Office
Rm.1207, King's garden 3 Block, 34, Sajik-ro 8-gil, Jongno-gu, Seoul 03174, Korea
Tel: +82-2-730-1993    Fax: +82-2-730-1994    E-mail: neonate2002@hanmail.net                

Copyright © 2019 by The Korean Society of Neonatology. All rights reserved.

Developed in M2community

Close layer
prev next